The CSR 2X Hot Start Taq Master Mix is a convenient, ready-to-use PCR solution featuring compatibility with a wide range of DNA templates, including mouse, human, plasmid, and bisulfite-converted DNA. It leaves a 5′ adenine overhang, making it ideal for TA cloning. The CSR Hot Start Taq Master Mix offers exceptional value per unit in terms of cost. Hot-Start Technology Hot Start Taq DNA Polymerase contains an aptamer based Hot Start technology that prevents amplification during PCR setup and minimizes the amplification of primer-dimer and nonspecific products. Initial incubation at 94 - 95 °C is NOT required. The aptamer contains a 3’ cap that prevents its amplification and ensures no interference with cloning or any downstream analysis.    Catalogue Number Number of Reactions Volume (mL) M0602-005 50 1.25 M0602-020 200 5.0   Documents Product Information Sheet Quick Guide Sheet SDS Sheet Figure 1. Gel Dye Fronts. 20 µl of diluted CSR 2X Hot Start Taq Master Mix with Dye (1X) was loaded on a 1 % agarose gel and ran at 120 V for 30 minutes Specification Robust and reliable reactions Tolerates a wide range of templates Amplification confirmed up to 5 kb in PCR reactions The error rate in PCR is 2.2 x 10^-5 errors per nucleotide per cycle Hot-Start technology prevents the formation of non-specific products in PCR Included in the Kit CSR 2X Hot Start Taq Master Mix GC Extender (For GC-rich or difficult to amplify templates) Protocol For Research Use Only. Not for use in diagnostic procedures.

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The CSR 2X Taq Master Mix is a convenient, ready-to-use PCR solution featuring compatibility with a wide range of DNA templates, including mouse, human, plasmid, and bisulfite-converted DNA. It leaves a 5′ adenine overhang, making it ideal for TA cloning. The CSR Taq Master Mix offers exceptional value per unit in terms of cost.     Catalogue Number Number of Reactions Volume (mL) M0501-005 50 1.25 M0501-020 200 5.0   Documents Product Information Sheet Quick Guide Sheet SDS Sheet Figure 1. Gel Dye Fronts. 20 µl of diluted CSR 2X Taq Master Mix with Dye (1X) was loaded on a 1 % agarose gel and ran at 120 V for 30 minutes Specification Robust and reliable reactions Tolerates a wide range of templates Amplification confirmed up to 5 kb in PCR reactions Included in the Kit CSR 2X Taq Master Mix GC Extender (For GC-rich or difficult to amplify templates) Protocol For Research Use Only. Not for use in diagnostic procedures.

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The CSR High Fidelity DNA Polymerase is a novel recombinant polymerase that contains a 5’ -> 3’ DNA polymerase and a 3 ‘ -> 5’ exonuclease domains along with a Sso7D tethering domain. The High-Fidelity DNA polymerase achieves 55-fold higher fidelity than Taq DNA polymerase and generates blunt end PCR products. The High-Fidelity DNA polymerase can generate DNA fragments up to 10 Kbp in length with higher accuracy and two-to-three-fold greater processivity than Taq DNA polymerase. Source High Fidelity DNA Polymerase is a recombinant polymerase derived from the archaeon Pyrococcus furiosus.    Catalogue Number Units M0401-002 200 U M0401-010 1000 U M0401-050 5000 U   Documents Product Information Sheet Quick Guide Sheet SDS Sheet Specification High fidelity amplification (> 55X Higher than Taq) Robust and reliable amplification Tolerates a wide range of templates 2-3 X faster speeds in PCR reactions (15 - 30 sec/ kb) Resistant to contaminated templates, such as plant DNA Amplification confirmed up to 10 kb in PCR reactions Superior performance for a broad range of templates (High AT to high GC rich) Included in the Kit CSR High Fidelity DNA Polymerase GC Extender (For GC-rich or difficult to amplify templates) 5X High Fidelity PCR Buffer Protocol For Research Use Only. Not for use in diagnostic procedures.

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The CSR Hot Start Taq DNA Polymerase reliably amplifies a wide variety of DNA templates—including mouse, human, plasmid, and bisulfite-converted DNA—producing consistent PCR products. This polymerase leaves a 5′ adenine overhang, making the products well-suited for TA cloning. Verified extension capabilities reach up to 5,000 base pairs, with speeds of up to 1 kb per minute. Hot-Start Technology Hot Start Taq DNA Polymerase contains an aptamer based Hot Start technology that prevents amplification during PCR setup and minimizes the amplification of primer-dimer and nonspecific products. Initial incubation at 94 - 95 °C is NOT required. The aptamer contains a 3’ cap that prevents its amplification and ensures no interference with cloning or any downstream analysis.    Catalogue Number Units M0202-010 1000 U M0202-050 5000 U M0202-500 50,000 U   Documents Product Information Sheet Quick Guide Sheet SDS Sheet Specifications Generates PCR product with 3'-dA overhangs The error rate in PCR is 2.2 x 10^-5 errors per nucleotide per cycle Generates PCR product of up to 5 kb Aptamer based Hot Start provides a reversible inhibition of non-specific PCR products Applications Effective in routine and high throughput PCR Incorporates modified nucleotides in PCR. Effective in DNA labeling. Kit Components 10X PCR Buffer 50 mM Magnesium Sulfate GC Extender (For GC-rich templates and difficult templates) Protocol For Research Use Only. Not for use in diagnostic procedures.

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The CSR ROBUST DNA Polymerase is a highly efficient next-generation DNA polymerase offering enhanced processivity and yield compared to Taq DNA polymerase. This enzyme enables shorter extension times and fewer cycles to achieve equivalent levels of amplification. It also features significantly improved resistance to various PCR inhibitors, chemical denaturants, and increased thermal stability, maintaining activity even after 1 hour at 95 °C. Its enhanced robustness allows for the use of lower-quality template DNA and simplifies DNA purification protocols. The ROBUST DNA Polymerase utilizes aptamer-based hot start technology, providing reversible inhibition that prevents primer-dimer formation and non-specific amplification, enabling reaction setup at room temperature. Hot-Start Technology The CSR ROBUST DNA Polymerase contains an aptamer based Hot Start technology that prevents amplification during PCR setup and minimizes the amplification of primer-dimer and nonspecific products. Initial incubation at 94 - 95 °C is NOT required. The aptamer contains a 3’ cap that prevents its amplification and ensures no interference with cloning or any downstream analysis.    Catalogue Number Units M0301-002 200 U M0301-010 1000 U M0301-050 5000 U   Documents Product Information Sheet Quick Guide Sheet SDS Sheet Specification Robust and reliable reactions Compatible with contaminated templates, such as plant DNA. Compatible with direct colony PCR.  2-3 X faster speeds in PCR reactions (15 - 30 sec/ kb) Tolerates a wide range of templates Amplification confirmed up to 5 kb in PCR reactions The error rate in PCR is 2.2 x 10^-5 errors per nucleotide per cycle Hot Start technology prevents the formation of non-specific products in PCR Included in the Kit CSR ROBUST DNA Polymerase 50 mM Magnesium Sulfate GC Extender (For GC-rich or difficult to amplify templates) Protocol For Research Use Only. Not for use in diagnostic procedures.

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The CSR Taq DNA reliably amplifies a wide variety of DNA templates—including mouse, human, plasmid, and bisulfite-converted DNA—producing consistent PCR products. This Taq polymerase leaves a 5′ adenine overhang, making the products well-suited for TA cloning. Verified extension capabilities reach up to 5,000 base pairs, with speeds of up to 1 kb per minute.      Catalogue Number Units M0101-010 1000 U M0101-050 5000 U M0101-500 50,000 U   Documents Product Information Sheet Quick Guide Sheet SDS Sheet Specifications Generates PCR product with 3'-dA overhangs The error rate in PCR is 2.2 x 10^-5 errors per nucleotide per cycle Generates PCR product of up to 5 kb Applications Effective in routine and high throughput PCR Incorporates modified nucleotides in PCR.  Effective in DNA labeling.  Kit Components 10X PCR Buffer 50 mM Magnesium Sulfate GC Extender (For GC-rich templates and difficult templates) Protocol For Research Use Only. Not for use in diagnostic procedures.

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