CSR Hot Start Taq DNA Polymerase, with 10X Reaction Buffer (no dNTPs)

Description

The CSR Hot Start Taq DNA Polymerase reliably amplifies a wide variety of DNA templates—including mouse, human, plasmid, and bisulfite-converted DNA—producing consistent PCR products. This polymerase leaves a 3′ adenine overhang, making the products well-suited for TA cloning. Verified extension capabilities reach up to 5,000 base pairs, with speeds of up to 1 kb per minute.


Hot Start Technology

Hot Start Taq DNA Polymerase contains an aptamer based Hot Start technology that prevents amplification during PCR setup and minimizes the amplification of primer-dimer and nonspecific products. Initial incubation at 94 - 95 °C is NOT required. The aptamer contains a 3’ cap that prevents its amplification and ensures no interference with cloning or any downstream analysis.

 

 Catalogue Number Units Number of Reactions
M0202-010 1000 U 400
M0202-050 5000 U 2000
M0202-500 50,000 U 20,000

 

Documents

  1. Product Information Sheet
  2. Quick Guide Sheet
  3. SDS Sheet
Specifications
  • Generates PCR product with 3'-dA overhangs
  • The error rate in PCR is 2.2 x 10^-5 errors per nucleotide per cycle
  • Generates PCR product of up to 5 kb
  • Aptamer based Hot Start provides a reversible inhibition of non-specific PCR products
  • Stable at room temperature for up to 2 weeks. 
Applications
  • Effective in routine and high throughput PCR
  • Incorporates modified nucleotides in PCR.
  • Effective in DNA labeling.
Kit Components
  • 10X PCR Buffer
  • 50 mM Magnesium Sulfate
  • GC Extender (For GC-rich templates and difficult templates)
  • Protocol

For Research Use Only. Not for use in diagnostic procedures.


CSR Hot Start Taq DNA Polymerase, with 10X Reaction Buffer (no dNTPs)

Product form

The CSR Hot Start Taq DNA Polymerase reliably amplifies a wide variety of DNA templates—including mouse, human, plasmid, and bisulfite-converted... Read more

SKU: CSR00002|M0202-010

$102.00

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    • A Certificate of Analysis (COA) is included with all products.

    Description

    The CSR Hot Start Taq DNA Polymerase reliably amplifies a wide variety of DNA templates—including mouse, human, plasmid, and bisulfite-converted DNA—producing consistent PCR products. This polymerase leaves a 3′ adenine overhang, making the products well-suited for TA cloning. Verified extension capabilities reach up to 5,000 base pairs, with speeds of up to 1 kb per minute.


    Hot Start Technology

    Hot Start Taq DNA Polymerase contains an aptamer based Hot Start technology that prevents amplification during PCR setup and minimizes the amplification of primer-dimer and nonspecific products. Initial incubation at 94 - 95 °C is NOT required. The aptamer contains a 3’ cap that prevents its amplification and ensures no interference with cloning or any downstream analysis.

     

     Catalogue Number Units Number of Reactions
    M0202-010 1000 U 400
    M0202-050 5000 U 2000
    M0202-500 50,000 U 20,000

     

    Documents

    1. Product Information Sheet
    2. Quick Guide Sheet
    3. SDS Sheet
    Specifications
    • Generates PCR product with 3'-dA overhangs
    • The error rate in PCR is 2.2 x 10^-5 errors per nucleotide per cycle
    • Generates PCR product of up to 5 kb
    • Aptamer based Hot Start provides a reversible inhibition of non-specific PCR products
    • Stable at room temperature for up to 2 weeks. 
    Applications
    • Effective in routine and high throughput PCR
    • Incorporates modified nucleotides in PCR.
    • Effective in DNA labeling.
    Kit Components
    • 10X PCR Buffer
    • 50 mM Magnesium Sulfate
    • GC Extender (For GC-rich templates and difficult templates)
    • Protocol

    For Research Use Only. Not for use in diagnostic procedures.


    Customer Reviews

    Based on 1 review
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    M
    Muhammad Hassan
    Phenomenal product at a great price!

    Product worked exactly as described. Will be buying again!

    Frequently Asked Questions

    Why is the ROBUST DNA polymerase called "ROBUST"?

    DNA polymerases are enzymes responsible for synthesizing new DNA strands during PCR. They do this by adding nucleotides to a primer attached to a template DNA strand. The ROBUST DNA polymerase is designed for challenging PCR conditions, featuring enhanced processivity, improved resistance to PCR inhibitors, thermal stability and hot start technology. It can amplify from lower-quality templates and allows for reduced cycle times.

    What is the significance of the 3' adenine overhang produced by some of these DNA polymerases?

    The 5' adenine (A) overhang produced by ROBUST and TAQ DNA polymerases is crucial for a cloning method called TA cloning. In TA cloning, the PCR product is directly ligated into a vector with a complementary 3' thymine (T) overhang. The matching 'A' and 'T' overhangs facilitate efficient ligation, creating recombinant plasmids with the PCR product inserted into the vector.tent

    What is processivity, and how does the ROBUST and High Fidelity DNA polymerases compare to Taq DNA Polymerase in this aspect?

    Processivity refers to the ability of a DNA polymerase to synthesize a long chain of DNA without detaching from the template. High processivity enables longer amplicons to be generated more quickly and with less opportunity for non-specific extension to occur. The ROBUST and High Fidelity polymerases have enhanced processivity compared to standard Taq polymerase, meaning they can synthesize longer DNA fragments more efficiently.

    What is 'Hot Start' technology and how does it benefit PCR amplification with Hot Start Taq Polymerase?

    Hot Start technology, in the context of Hot Start Taq Polymerase, uses an aptamer that has a 3’ cap to prevent amplification during PCR setup. The aptamer is a short single-stranded DNA or RNA molecule that binds specifically to the polymerase, blocking its activity until the high-temperature denaturation step. This prevents mis-priming and the formation of primer-dimers and other non-specific amplification products, resulting in more accurate and reliable PCR reactions. This means you don't need to initially incubate at 94 - 95 °C.

    What is the purpose of GC Extender, and when should it be used?

    GC Extender is an optional component intended to be used when amplifying difficult templates, such as those with high GC content (greater than 60%) or templates longer than 2.5 kbp. The precise volume needed (5µL – 15µL) should be optimized for each template and primer set, which helps the polymerase to efficiently amplify through complex regions and longer sequences of DNA.

    What considerations should be made when optimizing a PCR reaction using these polymerases?

    Optimization is crucial when using these polymerases, which include optimizing primer annealing temperature, the amount of template DNA, the concentrations of primers, the amount of DNA polymerase added, and magnesium sulfate concentration. The optimal number of cycles and the extension time also depend on your template. Using GC Extender can be optimized to a specific amount needed for difficult templates.

    What is High Fidelity DNA Polymerase and what makes it different from standard DNA polymerases like Taq and ROBUST?

    High Fidelity DNA Polymerase is a recombinant enzyme designed for accurate amplification of DNA. It's unique because it possesses a 5’ to 3’ DNA polymerase activity, a 3’ to 5’ exonuclease domain (which allows it to proofread and correct errors during DNA synthesis), and an Sso7D tethering domain that increases processivity. Compared to Taq DNA polymerase, it boasts 55-fold higher fidelity, generates blunt-end PCR products, can amplify larger DNA fragments (up to 10 kbp) with greater accuracy, and has two to three times the processivity.

    What are blunt end PCR products?

    Blunt end PCR products are double-stranded DNA fragments generated by PCR in which both strands end at the same nucleotide position, resulting in flush or blunt ends with no single-stranded overhangs. In other words, the 5′ and 3′ termini of each strand line up exactly, creating a perfectly straight (blunt) edge on both ends of the DNA fragment.

    Are all Master Mixes formulated for the same performance?

    No, the CSR 2X Taq Master Mix does not contain any special additives, making it suitable for standard PCR with minimal, if any, adjustments to your existing protocols. However, buffer and polymerase compositions vary between manufacturers and may perform differently under challenging PCR conditions, such as high-GC templates, impure DNA preparations, or the presence of low levels of PCR inhibitors. In such cases, optimal conditions for one master mix may not translate directly to another. If issues arise, it is recommended to perform independent optimization for the 2X Taq Master Mix.

    Why doesn't CSR 2x Taq Master or CSR 2X Hot Start Taq Mix work when I use my existing PCR protocol?

    There are three likely reasons.

    1. PCR Enhancer Reagent: If your PCR conditions require a PCR Enhancer Reagent to improve amplification efficiency, determine the optimal amount by titrating in 5 µL increments, up to a maximum of 20 µL per 50 µL reaction.
    2. Annealing Temperature: If no amplification product is visible, try reducing the annealing temperature by 3°C. If no band appears after a total adjustment of 7°C, determine the optimal annealing temperature by testing a range from the primer's calculated melting temperature (Tm) down to 7°C below it. For primers with different Tm values, use the lower Tm as the reference point.
    3. Cycle Number: If no band is visible, increase the number of cycles in increments of 3. 2X Taq Master Mixes work effectively between 25 and 40 cycles.

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